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Research Assistant

Lima

  • Organization: CIP - International Potato Center
  • Location: Lima
  • Grade: Administrative support - Administrative Services and Support - Generally no need for Higher Education
  • Occupational Groups:
    • Administrative support
    • Scientist and Researcher
  • Closing Date: Closed

Activities 1st stage:

-    Extraction of total nucleic acids and siRNAs from 150 samples of potato/sweetpotato germplasm for sRSA validation and samples for            interlaboratory testing for virus / viroid detection by sRSA

-       Preparation of libraries of the 150 samples extracted and of adequate quality for NGS analysis.

Shipment of the total libraries (150 samples) to be sequenced by Fasteris

Continue with the scheme of validation of PCR & RT-PCR for the detection of potato virus/viroid

Fortnightly reports of the progress of each activity detailed in this 1st stage

1 report halfway through the contract (reporting the fulfillment of activities of the 1st stage)

 

Activities 2nd stage:


Bioinformatic analyses of sequencing data of potato/sweetpotato germplasm samples for sRSA validation and samples for inter-laboratory testing of virus/viroid by sRSA (includes verification by PCR of the virus (s) reported by NGS, of the quality of each library, design of primers for specific detection of new virus variants, identification of possible causes of inconsistencies between samples and recommendations).

Conclude with the validation of PCR & RT-PCR for the detection of potato virus / viroid

Fortnightly reports of the progress of each activity detailed this 2nd stage

1 report at the end of the contract (reporting the fulfillment of activities of the 2nd stage

1 final report at the end of the contract (reporting the fulfillment of activities of 1st & 2nd stage)


Requirements

·         Biologist or Agronomist with excellent experience in Molecular Plant Pathology.

·         MSc degree on Molecular biology is desirable

  • 02-year experience on molecular techniques (RNA extraction, PCR, RT-PCR, cloning, sequencing, etc.) is a critical requisite
  • Demonstrated knowledge on the use of bioinformatics tools /softwares (Vector NT1 v.10, DNAsis, Velvet, MAC, etc.) are required
  •  02-year experience in collecting and managing plant pathogens at laboratory and greenhouse level.
  •  Experience on handling and maintenance of plants at greenhouse level is highly desirable.
  • Excellent interpersonal and team work skills.
  • Demonstrated ability on problem solving and capability to work independently.
  • Hard working with ability to handle multiple work activities in an effective and satisfactory manner.
  • Excellent Spanish written and verbal communication skills and intermediate English skills are required.
  • Knowledge of Microsoft office (Excel, Word and PPT as the basics)


Benefits

This position is a temporarily replacement and the employment contract will be for 3.5 months with the possibility of renewal. CIP prides itself on its collegial and supportive working environment, which allows space for personal and professional growth.



This vacancy is now closed.
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